Africans, along with the Spaniard Lucas Vásquez de Ayllón, were the first to settle what is today Jamestown, Virginia. Ayllón explored the eastern coast of Virginia in the early 1500s and was the first to bring Africans into what is now the United States. Ayllón found San Miguel de Guadalupe, a colony that flourished until 1527, when Ayllón died and was replaced by a more repressive ruler. Eventually the African rebelled, burning the settlement and forcing the Spanish to retreat to Haiti. Many of the Africans who fled the Spanish settlement established their own colony in the area. One cold say, then, that this community of Africans was, after those of the Native Americans, the first permanent colony in Virginia.
Note: All excerpts borrowed from 1001 Things Everyone Should Know About African American History by Jeffrey C. Stewart, solely for the intents and purposes to inform and educate.